Composite

Part:BBa_K5477030:Design

Designed by: Isaiah Kate Malana Escobar   Group: iGEM24_UCopenhagen   (2024-09-26)


XRE-pMEL1-NanoLuc reporter module


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 59
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 59
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 59
    Illegal BamHI site found at 3
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 59
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 59
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The pUUS plasmid is a multi-copy plasmid used in yeast, carrying a URA3 marker for selection in uracil auxotrophic strains. In our system, pUUS is utilized in the reporter module, enabling the expression of the reporter gene, NanoLuc.



Source

Homo sapiens and Saccharomyces cerevisiae

References